Thymidine Kinase Activity in Dictyostelium discoideum

Thymidine kinase activity in Dictyostelium discoideum.

Thymidine kinase activity is present in soluble and mitochondrial fraction of axenically grown cells of Dictyostelium discoideum. The overall activity does not change significantly during development.

Mutations Affecting Thymidine Metabolism in Dictyostelium discoideum

The tmpA600 mutation confers thymidylate synthase deficiency and thymidine auxotrophy to Dictyostelium discoideum. The tdrA6OO mutation enhances transport of thymidine and thereby reduces the auxotrophic requirement of tmpA600 strains. The tmpA locus maps to linkage group 111. The tdrA600 mutation is dominant and cosegregates with both linkage groups IV and VI, possibly because of a translocati...

Control of SCAR activity in Dictyostelium discoideum.

The WASP (Wiskott-Aldrich syndrome protein)/SCAR (suppressor of cAMP receptor) family of adaptor proteins regulate actin polymerization by coupling Rho-family GTPases to the activation of the Arp2/3 complex. SCAR exists within a complex of proteins, including Nap1 (Nck-associated protein 1), PIR121 (p53-inducible mRNA 121), Abi2 (Abl-interactor 2) and HSPC300. This complex was first reported to...

Dictyostelium discoideum

Dictyostelium discoideum is a cellular slime mold [4] that serves as an important model organism [5] in a variety of fields. Cellular slime molds have an unusual life cycle. They exist as separate amoebae, but after consuming all the bacteria in their area they proceed to stream together to form a multicellular organism. These features make them a valuable tool for studying developmental proces...

influx in Dictyostelium discoideum